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. 2017 Nov 1;33(Suppl 1):S-70–S-80. doi: 10.1089/aid.2017.0159

FIG. 1.

FIG. 1.

RANTES-PE38 depletes CCR5+ cells in vitro but not in vivo. (A, B) Outline of the macaque RANTES fusion protein construct, with RANTES and the Histidine tag flanking PE38 (A), and evaluation of protein purification through Coomassie staining and Western blot (B). (C, D) All LPLs (C) or CD3+ lymphocytes (D) after incubation for 40 h with 0, 10, or 50 nM of toxin. (E) Live cell counts after incubation of CHO-K1 cells or CHO-K1 cells stably transfected with CCR5 expression vector with RANTES-PE38 for 48 h. (F) Annexin V staining of a mixture of CCR5-expressing and unmodified CHO-K1 cells after a 48-h incubation with RANTES-PE38. (G, H) CCR5 expression on CD3+CD4+ PBMCs (G) or LPLs (H) of 1.5-year-old rhesus macaques treated with 1.25 mg/kg of RANTES-PE38. LPLs, lamina propria lymphocytes; PBMCs, peripheral blood mononuclear cells.