Fig. 2.

EZH2 suppression in GBM cells switches microglia polarization toward M1 phenotypes. a Murine primary microglia (PM) were co-cultured with GL261 cells for 24 h, and mRNA expression of multiple cytokines of microglia were determined by real-time PCR. b GL261 cells were treated with siEZH2 and DZNep for 24 h and then co-cultured with PM cells for another 24 h. Next, mRNAs of several M1 markers of microglia were tested by qPCR. c GL 261 cells were treated similar to b. Then, NO production in supernatant of the co-culture system was defined using the Griess assay. The amount of nitrite was normalized to untreated PM. d GL261 cells were treated similar to b, and mRNAs of multiple M2 markers were tested by qPCR. NC and PBS were set as controls, respectively, and nothing were added to mock group. All these experiments were repeated thrice. *p < 0.05, compared with corresponding control group