Fig. 6.

GBM cells with EZH2 inhibition can promote microglia proliferation with TGFβ2 dependent. a Murine GL261 cells were treated with siEZH2 and DZNep for 24 h and then co-incubated with primary microglia for another 24 h. Next, microglia proliferation was determined by CCK-8 assays. b Primary microglia were incubated with cytokines TGFβ1, TGFβ2, and 1400 W with working concentrations of 10 μg/ml, 10 μg/ml, and 500 uM. Twenty-four hours later, cell proliferation was analyzed by CCK-8 assays. c Murine GL261 cells were treated with siEZH2 and DZNep for 24 h and then co-incubated with primary microglia for another 24 h. Next, GBM were removed and TGFβ2 antibodies were added into microglia at the concentration of 10 μg/ml for 24 h. Next, microglia proliferation was analyzed by CCK-8 assays. PBS and NC siRNAs were set as controls for TGFβ2 antibodies and siEZH2, respectively, and nothing were added to CON group. All these experiments were repeated thrice. *p < 0.05