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Evaluation of cell viability. ATP levels were measured in sandwich‐cultured human hepatocytes following exposure to increasing concentrations (0.1, 0.316, 1.0, 3.16, 10, 31.6, 100 μmol/L) of CDCA (A), OCA (B), tauro‐OCA (C), glyco‐OCA (D), and positive controls (Tamoxifen 50 μmol/L and Aflatoxin 10 μmol/L) for 72 h. The data represent means from triplicate wells from 1 donor.
