Figure 4. CHCHD4P4 overexpression. HK-2 cells were incubated with pcDNA3.1 encoding CHCHD4P4 cDNA (A) for 6 h for a stable transfection. HK-2 cells were treated with pcDNA3.1 or pcDNA3.1-CHCHD4P4 combined with calcium oxalate monohydrate (200 µg/mL) for 24 h. mRNA levels of the epithelial-mesenchymal transition (EMT) markers in the stable HK-2 cell clones (B). Protein levels (D) and immunofluorescence microscopic analysis (C) of the EMT markers in the indicated HK-2 cell clones. Scale bars = 100 µm. Data are reported a means±SD. *P<0.05, **P<0.01, ***P<0.001 (Student's t-test).
