Figure 5. CHCHD4P4 silencing. HK-2 cells were transfected with the transfection agent (A) without siRNA (mock) or with siRNA against CHCHD4P4. mRNA levels (B), protein levels (D), and the results of the immunofluorescence microscopic analysis (C) of the EMT markers in the indicated HK-2 cells that were transfected with the siRNA against CHCHD4P4. Scale bars = 100 µm. Data are reported a means±SD. *P<0.05, **P<0.01 (Student's t-test).