Figure 6. Effect of CHCHD4P4 on the cell cycle of HK-2 cells. Immunofluorescence microscopic analysis of 5-ethynyl-2'-deoxyuridine (EDU) (A) in the indicated HK-2 cell clones. Scale bars = 100 µm. Blue represents the nucleus, and red indicates the proliferation of cells. The CCK8 assay (B) was used to assess HK-2 cell proliferation (***P<0.001 vs pcDNA3.1, # ##P<0.001 vs CaOx+pcDNA3.1, t-test). Annexin V flow-cytometric analysis of the HK-2 cells that were transfected with the pcDNA3.1 encoding CHCHD4P4 cDNA for 6 h and treated with calcium oxalate monohydrate for 24 h (C). Cell cycle analysis of the HK-2 cell clones (D).
