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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: J Immunol. 2017 Oct 16;199(10):3634–3643. doi: 10.4049/jimmunol.1700820

Fig. 6. Eicosanoid signaling acts downstream of LPS to amplify insulin activity.

Fig. 6

(A) Survival of DMSO or indomethacin treated mice after challenge with LPS (2.5 μg/kg) and insulin. (B and C) Core temperatures (B) and blood glucose concentrations (C) of mice 1 h after challenge as described in (A). (D - G) Phosphorylation of liver AKT (D, F) and insulin receptor (E, G) determined by Western blot 1 h after challenge as described in (A). (H) Plasma insulin concentrations of mice 1 h after treatment as in (B). (I) Plasma prostaglandin concentrations in mice 1h after challenge with DBPS, LPS (400 μg/kg), insulin, or LPS and insulin. (J) P values for the indicated comparisons of data from (J) as determined by ANOVA and Tukey's multiple comparisons test. (K) Survival of mice challenged with insulin and LPS (10 μg/kg). Data are pooled from at 3 (A, K) or 2 experiments (B – I). Error bars, mean ± SD. Statistically significant differences were determined by a 2-tailed unpaired t-test; * P ≤ 0.05.