Figure 2.

Stroma altered target proteins and the sensitivity of AML to ABT737. The pathways affected by ABT737 treatment are circled in the pie charts on the left. The bar graphs display proteins whose expression was significantly altered in (A) monocultured samples treated with ABT737 versus untreated control (n = 20) and (B) co-cultured samples treated with ABT737 versus untreated control (n = 20). The numbers on the left are the numbers of the pathways (as indicated on the pie chart and in the Online Supplementary Figure S1). The calculation of the black, white, and gray area and the color key on the bar graphs are described in the legend for Figure 1. (C) Bar graph displays ABT737-induced specific apoptosis for samples cultured alone and co-cultured with stroma. The sample groupings and the statistical calculation of apoptosis are described in the Online Supplementary Materials and Methods. (D) Box and whisker plots display differences in protein expression between untreated samples in groups I and III with and without stroma (top panel) and protein expression at the baseline level of untreated samples in groups I and III in co-culture (bottom panel). Whiskers indicate the range from minimum to maximum values. The line in the middle of the box is plotted at the median.