Figure 4.

β-LGND2 regulates distinct gene expression program in the liver of mice fed with HFD. Male C57BL/six mice (n = 5/group) were maintained on a normal diet (CON) or high fat diet (HFD) and treated with vehicle or 30 mg/kg/day s.c. β-LGND2 for 10 weeks. Mice were sacrificed and liver sections were snap frozen. RNA was isolated and expression of genes was measured by next-generation sequencing (n = 3/group). Genes that were statistically significantly (q < 0.05) regulated by β-LGND2 compared to vehicle-treated HFD-fed mice are represented in panel (a). (b) Ingenuity pathway analysis with statistically significant genes (q < 0.05) was performed to determine the pathways regulated by β-LGND2 in liver. (c) Genes that are differentially regulated by β-LGND2 (q < 0.05) and belonging to the PXR pathway are represented. (d) Genes (q < 0.05) that are important for lipogenesis (left) and clearance of lipids (right) are represented. In panel (d) left figure, b > a and c at p < 0.05, while a and c are not statistically different. In panel (d) right figure, b < a and c at p < 0.05, while a and c are not statistically different. Values are expressed as average ± SE