Figure 2.

In silico modelling of the uniqueness of alignments of simulated short-read sequences to the region of Chromosome 22 containing CYP2D6, CYP2D7 and CYP2D8 (hg19, chr22:42,518,000-42,555,000). Simulated singleton reads (a) and paired-end reads (b) from 50 to 5,000-nt in length were generated from this region. For paired-end reads, insert lengths varied from 300 to 800-nt. Exons, introns and genomic features to which reads mapped uniquely with GSNAP are shown as green‘1’; regions to which reads did not map uniquely are shown as red ‘0’.