Fig. 3.

Tan IIA upregulates CYP3A4, MDR1, GST and GSTM1 expression in H₂O₂ treated HUVECs via PXR activation. Cells were transfected with PXR siRNA, with or without pretreatment with Tan IIA for 24 h then treatment with 400 μM H₂O₂ for another 3 h. RIF treatment was used as a positive control. (A-D) CYP3A4, MDR1, GST and GSTM1 mRNA expression was measured by qRT-PCR, using GAPDH as an GAPDH was internal control. Data are expressed as percentages of control and are the mean ± SD of 3 replicates. ^*p<0.05, ^**p<0.01, ^***p<0.001 compared with H₂O₂ group.