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. 2017 Jun 26;2(3):229–240. doi: 10.1016/j.jacbts.2017.02.004

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Presence of Individual PC-OxPL Species in Valve Leaflets

Liquid chromatography-tandem mass spectrometry analysis of the most abundant fragmented phosphocholine-containing oxidized phospholipid (PC-OxPLs) compounds extracted from human stenotic aortic valves and represented as (A) individual PC-OxPL compounds or (B) as total PC-OxPL levels within each of the valves. Reverse phase separation coupled with tandem mass spectrometry detection was used to detect POVPC (1-palmitoyl-2-[5'-oxo-valeroyl]-sn-glycero-3-phosphocholine), PGPC (1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine), PONPC (1-palmitoyl-2-[9'-oxononanoyl]-sn-glycero-3-phosphocholine), KODiA-PC (1-[palmitoyl]-2-[5-keto-6-octene-dioyl]-sn-glycero-3-phosphocholine), and PAzPC (1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine).