Table 2.
SEC-SAXS statistics.
Construct | Rg (Å) | Monomer MW | SAXS MoW (Oligomeric Species) | Chi2 | NSD |
---|---|---|---|---|---|
CC60-185 | 60.2 | 14.6 kDa | 72 kDa (4.9-mer) | 0.9692 | 0.900 |
CC69-185 | 56.5 | 13.7 kDa | 49 kDa (3.6-mer) | 1.104 | 0.695 |
1.181 (P2) | 1.532 (P2) | ||||
CC33-111 | 29.4 | 8.8 kDa | 25 kDa (2.8-mer) | 1.006 | 0.606 |
CC112-185 | 32.9 | 8.9 kDa | 21 kDa (2.4-mer) | 1.036 | 0.526 |
1.118 (P2) | 1.801 (P2) | ||||
mLZ93-171 | 32.2 | 9.1 kDa | 20 kDa (2.2-mer) | 1.072 | 0.586 |
1.078 (P2) | 1.268 (P2) |
Chi2 and normalized spatial discrepancy (NSD) values are from ab initio modeling. P2 indicates values obtained from ab initio modeling when constrained to P2 symmetry. Rg, radius of gyration; MoW= molecular weight calculated from SAXS.