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. 2017 Nov 14;12(11):e0188095. doi: 10.1371/journal.pone.0188095

Fig 2. High performance liquid chromatography (HPLC) chromatograms of the reaction mixtures of asparagine and sugars (black: 60°C, blue: 80°C, cyan: 100°C, red: 180°C).

Fig 2

A 20 μL aliquot of sample was injected and the wavelength used for detection was 200 nm. Triplicate samples were manually injected in the chromatographic system. Panel A: Fraction 2 from the reaction of asparagine with glucose. Panel B: Fractions 3–5 collected as discrete MRPs in the reaction of asparagine with glucose. Panel C: Fraction 2 from the reaction of asparagine with fructose. Panel D: Fractions 3–5 collected as discrete MRPs in the reaction of asparagine with fructose.