Fig 8. Live imaging of MDA-MB-231 cells on TCP, 1 kPa and 103 kPa PA gels immediately post-seeding on the substrate.

(A) Cells were harvested from TCP and seeded onto TCP. (B) Cells were harvested from TCP and seeded onto a 103 kPa gel. (C) Pre-adapted cells at P3 were harvested from a 103 kPa gel and seeded onto a 103 kPa gel. (D) Cells were harvested from TCP and seeded onto a 1 kPa gel. (E) Pre-adapted cells at P3 were harvested from a 1 kPa gel and seeded onto a 1 kPa gel. Scale bar is 100 μm.