Fig 9. MDA-MB-231 cell spreading area as a function of time and cell pre-adaptation to the substrate.

(A) Spreading area for cells seeded onto 1 kPa gel, where cells were either harvested from TCP (TCP→1) or pre-adapted on a 1 kPa gel for 3 passages (1→1). Cells harvested from TCP and seeded onto TCP were used as control (TCP→TCP). (B) Spreading area for cells seeded onto 103 kPa gel, where cells were either harvested from TCP (TCP→103) or pre-adapted on a 103 kPa gel for 3 passages (103→103). Cells harvested from TCP and seeded onto TCP were used as control (TCP→TCP). C) Cell spreading rate calculated as per Eq 5. *represent significant difference from all other conditions (p<0.05, n = 3).