Fig 10. Cell spreading area and circularity of MCF-7A cells as a function of adaptation to substrate stiffness.

Cells were seeded onto the PA substrates for 9 consecutive days without re-passaging. (A) Representative phase contrast images of cells on TCP and 1 kPa PA gels. Scale bar is 100 μm. (B) Cell spreading area and (C) circularity of cells on TCP and 1 kPa PA gels as a function of culture time.