Fig 11. Live imaging of MCF-7 cells on TCP and 1 kPa PA gels immediately post-seeding on the substrate.

(A) Cells were harvested from TCP and seeded onto TCP. (B) Cells were harvested from TCP and seeded onto a 1 kPa gel. (C) Pre-adapted cells at P3 were harvested from a 1 kPa gel and seeded onto a 1 kPa gel. Scale bar is 100 μm (A-C). (D) Spreading area for cells seeded onto 1 kPa gel, where cells were either harvested from TCP (TCP→1) or pre-adapted on a 1 kPa gel for 3 passages (1→1). Cells harvested from TCP and seeded onto TCP were used as control (TCP→TCP). (E) Cell spreading rate calculated as per Eq 5. *represent significant difference between all conditions (p<0.05, n = 3).