Fig 3. Treatment with eATP reduces nitric oxide production in HSV endothelial cells.

HSVEC (>70% confluence, passages 2–4) were either untreated (Ctrl) or treated with ATP (2 mM) or ATP and FCF (100 μM) for 2 hours. Cells were stimulated with carbachol (1 μM, CCH) for 10 min at 37°C, and nitric oxide (NO) generated by the cells was measured as nitrite. NO generated with CCH was set as 100%, n = 4 passages, *p < 0.05, (One way ANOVA).