Fig 5. Time course of phosphorylation of p-ERK1/2 MAPK in ATP treated HSVEC.

HSVEC were untreated (0) treated with ATP (2 mM) for various time points (10, 30, and 60 minutes) and phospho p44/42 MAPK (P-ERK1/2) and total p44/42 (ERK1/2) proteins were quantitated with immunoblotting (adjusted to the loading control GAPDH). (A) Representative western blot of phospho ERK1/2, (B) Cumulative data showing the phosphorylation (fold) of p ERK1/2 MAPK, * p < 0.05, N = 4, (One way ANOVA).