Fig 7. Effect of arginase inhibitor and L-arginine on eATP-induced reduction of nitric oxide production in HSV endothelial cells.

HSVEC were either untreated (Ctrl), treated with ATP (2 mM), L-NAME, (100 μM), ATP with L-arginine (L-Arg, 2 mM), or ATP with NOHA (10 μM), for 2 hours. The cells were then stimulated with carbachol (CCH, 1 μM) for 10 minutes and the nitric oxide generated was measured as nitrite using the NO assay kit and relative percent of NO generated was calculated. NO generated with CCH was set as 100%, n = 6 passages, *p < 0.05, (One way ANOVA).