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. 2017 Nov 14;12(11):e0188001. doi: 10.1371/journal.pone.0188001

Fig 2. qPCR examination of NDT80 dependent 3' transcriptional induction for representative genes with mid-sporulation specific internal TSS.

Fig 2

Wild-type (A14201) strain was synchronized in sporulation using β-estradiol inducible NDT80. The levels of 3' and 5' expression of control transcripts (CDC3 and CDC10) and transcripts of a subset of genes identified with mid-sporulation specific internal TSSs (MRK1, IQG1, DRS1 and SAD1) were measured by qPCR of samples harvested when β-estradiol was added to the media and 2 hours after NDT80 induction. The ratios of 3' expression to 5' expression are shown on a log2 scale. Values shown are the averages from two or more independent experiments. Error bars represent standard error of the mean. Statistical differences were examined by Student's t-test. Symbols denote statistical significance (*  =  p<0.05; ***  =  p<0.001; **** =  p<0.0001).