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. 2017 Jul 27;8(52):89539–89551. doi: 10.18632/oncotarget.19647

Figure 3. EGFR promoted YAP1 stabilization.

Figure 3

A. MG132 (10 μM, 3 h) induced YAP1 accumulation in A549 cells in the absence of EGF (lanes 1 and 2); while no further increase of YAP1 level induced by MG132 (3 h) in the presence of EGF (1 h) (lanes 3 and 4). CHX (20 μg/ml) treatment caused decreased YAP1 levels in A549 cells B. while YAP1 levels maintained in H1975 and HCC827 cells C. with CHX treatment. D. By immunoprecipitating YAP1, its binding with β-TRCP was detected in A549 cells. EGF treatment reduced the binding between YAP1 and β-TRCP. E. In the presence of gefitinib (0.1 μM, 24 h), enhanced YAP1/β-TRCP binding was detected in HCC827 cells.