Figure 4. Sun2 knockdownpromotes the prostate cancer growth.

(A) Western blot was used to confirm the sh-Sun2 transfection efficiency. (B) Cell proliferation levels were measured using the cell counting kit-8 at 1, 2, 3 and 4 days. (C) and (D) Cell-cycle distribution and cell apoptosis were analyzed by flow cytometry, respectively. (E) Tumorigenicity assay in nude mice was performed in Sh-Sun2 downexpression stable cell lines vs.control. *P < 0.05, **P < 0.01.