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. 2017 Sep 20;8(52):89707–89721. doi: 10.18632/oncotarget.21111

Figure 5. BMI1 decreases HU-elicited S-phase arrest.

Figure 5

(A) MCF7 EV, BMI1, shCtrl, and shBMI1 cells were treated with nocodazole (0.1 μg/ml) without and with HU (0.1 mM) for 24 hours. Histone H3 S10 phosphorylation was determined using IF staining; nuclei were counter-stained with DAPI. Experiments were repeated 3 times; typical images from a single repeat are included (B). More than 8,000 nuclei from 12 randomly selected areas per slide were counted to determine the percentage of nuclei positive for histone H3 S10 phosphorylation. Means ± SD (standard deviation) were graphed; *p < 0.05 (two-tailed Student's t-test) in comparison to the individual mock treatments, #p < 0.05 in comparison to HU-treated EV cells; and $p < 0.05 in comparison to HU-treated shCtrl cells.