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MCF7-based EV, BMI1ΔNLS, BMI1ΔHT, BMI1ΔRF, and BMI1ΔPS stable lines have been previously established [31]. These lines were treated as indicated and examined for ATRpT1989, ATR, CHK1pS345, CHK1, actin, γH2AX, and H2AX. Experiments were performed twice. Typical images from a single repeat are shown.
