Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 20;8(52):89707–89721. doi: 10.18632/oncotarget.21111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2017 Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Cell lysate from the indicated MCF7 BMI1ΔRF, BMI1ΔNLS, BMI1ΔHT, or BMI1ΔPS cells (A–E) were pre-treated with benzonase at 1 U/μl on ice for 1 hour, followed by IP with a monoclonal anti-FLAG (M2) (A–D) or anti-ATR antibodies (E). Western blot analyses were performed for ATR, TOPBP1, and the indicated BMI1 mutants (using a polyclonal anti-FLAG antibody). 1/10 of cell lysates used for IP were also analyzed. Experiments were repeated at least three times; typical results from a single repeat are shown. (D) LS: long exposure; SS: short exposure.