Skip to main content
. 2017 Sep 22;8(52):89824–89836. doi: 10.18632/oncotarget.21158

Figure 2. CYP11A1 overexpression and androgen could induce autophagy in BeWo cell line.

Figure 2

(A) Western blot result shows that CYP11A1 overexpression induces LC-3II expression, while flutamide (10 μM) could inhibit the LC-3II expression. (B) Beclin 1 expression would also be induced after CYP11A1 overexpression, which could be blocked by androgen receptor (AR) inhibitor, flutamide. (C, D) P62 and Pink1 expression would also be induced by CYP11A1 expression. (E) Testosterone could induce LC-3II expression dose-dependently after 48 h treatment. (F) Overexpression of increasing amount (0.5 μg,1 μg,2 μg CYP11A1) plasmid induce CYP11A1 protein expression * means P < 0.05, ** means P < 0.01, *** means P < 0.001.