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. 2017 Sep 12;8(52):89887–89902. doi: 10.18632/oncotarget.20834

Figure 3. IMD-4482 inhibits adhesion and invasion of PAI-1-positive ovarian cancer cells (SKOV3ip1 and HeyA8 cells) but not PAI-1-negative cells (OVCAR3).

Figure 3

In vitro adhesion assay (A). A total of 1 × 105 ovarian cancer cells (left, SKOV3ip1; middle, HeyA8; right, OVCAR3) were plated onto vitronectin-, fibronectin-, collagen type I-, and laminin-coated 96-well plates. After incubation for 50 minutes at 37°C, plates were washed to discard non-adherent cells, and the relative number of attached cells was measured, Data represents mean ± SD, n = 5 from triplicate independent experiments. (B) Representative images of in vitro adhesion assay of SKOV3ip1 cells (B). Bar, 200 μm. In vitro invasion assay (C). A total of 4 × 104 SKOV3ip1 (left) and 8 × 103 HeyA8 (right) cells were plated on the top chamber in serum-free medium with IMD-4482, and allowed to invade for 48 hours. Invading cells on the underside of the filter were counted. Representative images are shown. Bar, 200 μm. Data represents mean ± SD, n = 5 from triplicate independent experiments. *; P < 0.05, **; P < 0.01, n.s.; not significant.