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. 2017 Sep 12;8(52):89887–89902. doi: 10.18632/oncotarget.20834

Figure 5. IMD-4482 suppressed proliferation and induced G0/G1 cell cycle arrest and apoptosis in PAI-1-positive ovarian cancer cells.

Figure 5

Effect of IMD-4482 on cell proliferation (A). SKOV3ip1, HeyA8, and OVCAR3 cells were plated onto 96-well plates and cultured in DMEM containing 2% FBS with or without IMD-4482. Effect of IMD-4482 on cell cycle distribution (B and C). Cells treated with or without IMD-4482 for 24 hours were stained with propidium iodine and analyzed by flow cytometry. Representative flow histograms (B), and percentages of cells in G0/G1, S, and G2/M phase (C) are shown. Western blot (D). Cells were incubated with or without IMD-4482 for 24 hours. Cell lysates were immunoblotted with antibodies against PARP, CDK2, cyclin D3, and p27kip1. β-actin was used as a loading control.