Figure 1. Intratumoral NK-92 cells up-regulate SESN2 and SESN3 expression.

(A) Representative flow cytometry plots showing CD45⁺CD56⁺ NK-92 cells in the spleens, peritoneal cavity and tumor xenografts. NK-92 cells were adoptively transferred into tumor-bearing mice through intraperitoneal injection and were then isolated from indicated tissues based on CD45 and CD56 expression. (B) SESN1, SESN2 and SESN3 protein levels in NK-92 populations isolated from different tissues. C: in vitro cultured NK-92 cells. Sp-NK: NK-92 cells isolated from spleens. Pe-NK: NK-92 cells isolated from peritoneal cavity. T-NK C56^hi: intratumoral NK-92 cells expressing high CD56. T-NK CD56^dim: intratumoral NK-92 cells expressing dim CD56. (C) Statistics for (B). N= 5 per group. ^*, p<0.05; ^***, p<0.001.