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. 2017 Oct 4;8(52):90132–90143. doi: 10.18632/oncotarget.21487

Figure 5. SESN2 and SESN3 inhibit NK-92 cell activity in vivo.

Figure 5

(A) Representative dot plot showing the expression of GFP and CD56 in Len-S2-transduced NK-92 cells isolated from peritoneal cavity after doxycycline pre-treatment and adoptive transfer. (B) Proportions of SESN2 or SESN3-overexpressing NK-92 cells and control NK-92 cells in total peritoneal cells on day 2 after adoptive transfer. V: vehicle. DOX: doxycycline. Len-C: NK-92 cells transduced with lentivirus containing no SESN sequence. Len-S2: NK-92 cells transduced with Len-S2. Len-S3: NK-92 cells transduced with Len-S3. N=3 per group. (C & D) Phosphorylated AMPKα (C) and mTOR (D) in transferred NK-92 cells was detected using flow cytometry. Left panels: representative histograms. Right panels: statistics. N=4 per group. (E) Expression of perforin (PFR) and granzyme B (GZMB) in transferred NK-92 cells. Left panel: representative dot plots. Right panel: statistics. N=5 per group. *, p<0.05; **, p<0.001; ***, p<0.001.