Table 1. Neratinib kills afatinib resistant H1975 clones and interacts with valproate to enhance tumor cell killing.

	VEH	NER	LAP	AFA	VEH	NER	LAP	AFA	VEH	NER	LAP	AFA	VEH	NER	LAP	AFA
% death	1	12	2	14	3	17	6	15	1	23##	2	5¶	10	39###	8	9
% death	1	14	2	19	2	26#	8	16	1	35##	3	6¶	10	50###	14	13
% death	1	17	2	12	2	18	7	29#	1	38##	6	3¶	13	61###	19	14
% death	1	24	8	13	2	28	10	11	1	30##	8	4¶	9	75###	18	7
% death	1	18	2	11	3	39#	14#	21#	1	25##	10	4¶	11	47###	16	15
VEH						VAL			VEH				VAL
Parental H1975 clones									Afatinib-R H1975 clones

Parental and afatinib resistant H1975 clones were treated with vehicle control, sodium valproate (250 μM), neratinib (0.5 μM), afatinib (0.5 μM), lapatinib (2.0 μM) or the drugs in combination as indicated in the Table. Twenty-four h after drug exposure, cells were treated with live/dead reagent and cell viability determined as described in the Methods. (n = 3 +/-SEM) ^# p < 0.05 greater than corresponding value in vehicle control cells; ^## p < 0.05 greater than corresponding value in parental H1975 clones; ^### p < 0.05 greater value than corresponding value in afatinib resistant clones; ¶ p < 0.05 less than corresponding value in parental H1975 clones