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. 2017 Oct 9;8(52):90278–90290. doi: 10.18632/oncotarget.21744

Figure 4. Immunoreactivities of membrane transporters and tight junction (TJ) molecules in AOM-induced colon carcinogenesis in rats.

Figure 4

(A) Whole-tissue view of colon adenoma tissues with adjacent normal colonic epithelia immunostained for the TJ molecule zona occludens-1 (ZO-1) or occludin (green) and E-cadherin (adherence junction marker as a reference; magenta). Arrows: strong signals of occludin. Scale bar, 1 mm. (B) High-power view of normal colonic epithelium or colon adenoma tissues double-immunostained with E-cadherin (magenta) and ZO-1 or occludin (green), with nuclear staining (blue). Yellow arrowheads: signals at the apical cell border. Red arrowheads: strong signals distributed in the lateral cell walls and apical cell border. Scale bar, 10 μm. (C) High-power view of normal colonic epithelium or colon adenoma tissues double-immunostained with E-cadherin (magenta) and for the membrane transporters organic anion transporting polypeptide 1B2 (OATP1B2) or sodium-taurocholate co-transporting polypeptide (NTCP) (green), with nuclear staining (blue). Scale bar, 10 μm. All two adenomas and two adenocarcinomas showed similar results.