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. 2017 Oct 9;8(52):90278–90290. doi: 10.18632/oncotarget.21744

Figure 6. ICG fluorescence imaging of macroscopically small proliferative lesions in AOM-induced colon carcinogenesis in rats after a 30-min ICG enema.

Figure 6

(A) Ex vivo images. Left panels: bright-field images. Middle panels: ICG fluorescence (green) images. Small proliferative lesions are indicated by arrows in both case 1 and case 2. Scale bar, 2 mm. Right panels: representative high-power views of small proliferative lesions with blood vessel staining (TL488; red). In case 1, both #1 and #2 were histologically diagnosed as aberrant crypt foci (ACF); in case 2, #1 was diagnosed as adenoma and #2 as focal mucosal hyperplasia. Scale bar, 100 μm. (B) Fluorescent WGA (blue) stained images of ICG+ (green) proliferative lesions in the case 2 of the panel (A). Blood vessels were stained with TL488 (red). Asterisk indicates the area where mucus was removed during staining procedure. Scale bar for the whole-tumor view, 2 mm; for the magnified view, 100 μm. (C) ACF double-immunostained with occludin (green) and E-cadherin (magenta), with nuclear staining (blue). Red arrowheads: strong signals distributed in the lateral cell walls and apical cell border. Scale bar, 20 μm. (D) In vivo imaging of ACF by fluorescence endoscopy. Ex vivo bright-field and ICG-fluorescence image and HE-stained section image corresponding to the endoscopic images. Arrow: ACF. Scale bar, 2 mm.