Table 1.
Experimental Parameters for Gliding Assay of Kinesin-5 Motors Against an Optical Trapping Force
| Data Set | Minimal MT Length (μm) | Maximal MT Length (μm) | No. of Traces | Total Time (s)a | Total Time in Pause (s)a | % in Pause |
|---|---|---|---|---|---|---|
| Eg5 | 9.1 | 25.8 | 14 | 432.4940 | 203.5535 | 47.1 |
| Cin8 5 nM | 6.9 | 24.4 | 22 | 624.0950 | 488.7475 | 78.3 |
| Cin8 10 nM | 5.7 | 18.3 | 16 | 479.9005 | 244.7835 | 51.0 |
| Cin8 25 nM | 6.5 | 23.9 | 54 | 982.5305 | 435.0395 | 44.3 |
| Cin8 50 nM | 5.3 | 29.3 | 38 | 856.7845 | 433.0980 | 50.5 |
| Cin8 negative | 7.6 | 23.9 | 21 | 276.3525 | 175.6170 | 63.5 |
| Cin8 positive | 5.3 | 29.3 | 109 | 2666.9580 | 1499.9355 | 56.2 |
| Cin8 0–185 nM μm | 5.7 | 24.4 | 41 | 1218.005 | 866.4400 | 71.1 |
| Cin8 185–370 nM μm | 5.7 | 14.7 | 26 | 587.1465 | 270.6450 | 46.1 |
| Cin8 370–555 nM μm | 8.0 | 19.6 | 33 | 625.3105 | 293.3445 | 46.9 |
| Cin8 555–740 nM μm | 11.4 | 23.9 | 15 | 277.8710 | 112.8460 | 40.6 |
a
Values are calculated from the total number of data points analyzed, acquired at 2 kHz.