Figure 3. Effects of the LRRC8A or LRRC8D RNAi knockdowns on swelling‐activated efflux of [³H]taurine (A) and d‐[¹⁴C]aspartate (B).

Astrocytic [³H]taurine and d‐[¹⁴C]aspartate release kinetics were measured simultaneously in cells preloaded with both radiotracers. Cells were transfected with negative control siRNA (siNC) or gene specific siLRRC8A, siLRRC8D_1, or siLRRC8D_2. For clarity, the results with [³H]taurine and d‐[¹⁴C]aspartate are presented in separate panels. Data are the means ± SEM of 6–7 independent experiments per group performed in 3 different astrocyte cultures. ^*** P < 0.001 maximal release values vs. siNC; ^### P < 0.001 integral 10‐min release values vs. siNC.