Table 1.

qPCR primers and siRNA constructs

Target gene	Catalogue no. for qPCR primers	siRNA name (catalogue no.)	siRNA target sequence
NC	N/A	AllStars NC (SI03650318)	Scrambled (proprietary)
LRRC8A	QT01575483	siLRRC8A_4 (SI01725360)	5′‐CTCTACCTGAACCGCAACAAA‐3′
LRRC8B	QT00434805	siLRRC8B_1 (SI01673007)	5′‐ATGGTCTCACGTCGTCCTATA‐3′
		siLRRC8B_2 (SI01673014)	5′‐CAGATAATCGTCAAAGTCATT‐3′
		siLRRC8B_4 (SI01673028)	5′‐CTGGCACAATAACATCGCTTA‐3′
LRRC8C	QT01583897	siLRRC8C_1 (SI01550815)	5′‐TTGGATGTACTTTACAAGTCA‐3′
		siLRRC8C_2 (SI01550822)	5′‐ACGGCCTGACGTGCCTTTATA‐3′
		siLRRC8C_4 (SI01550836)	5′‐TCCCGAGAAGTTCGTGGTTGA‐3′
LRRC8D	QT00370111	siLRRC8D_1 (SI01904287)	5′‐AGGCATTGAATCAAGACGTAA‐3′
		siLRRC8D_2 (SI01904294)	5′‐CAGCAGTACGTATTTATCAAT‐3′
		siLRRC8D_4 (SI01904308)	5′‐TGCCGTCGCCTGCAAATTCAA‐3′
LRRC8E	QT01591352	siLRRC8E_2 (SI01665146)	5′‐AACGATCAACATATATTTAAG‐3′
		siLRRC8E_3 (SI01665153)	5′‐ATGCCAAGTCCTTATGTTATA‐3′
		siLRRC8E_4 (SI01665160)	5′‐CAGCTTAGTTAGTTTCCTATA‐3′
GAPDH	QT00199633	N/A	N/A
RPL13a	QT00178675	N/A	N/A
RPS20	QT00431333	N/A	N/A

All validated qPCR primers and RNAi constructs were from Qiagen. N/A, not applicable; NC, negative control.