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. 2017 Oct 25;595(22):6869–6885. doi: 10.1113/JP275052

Figure 1. TRPV4 is expressed in retinal microvascular cells.

Figure 1

A, RT‐PCR. Trpv1, Trpv4 and Pecam‐1 but not Trpv2 & 3 transcripts are expressed in HrMVECs. B, Western blot. TRPV4 protein expression is prominent in HrMVECs but weak in HCECs. C, HrMVEC labelled by anti‐TRPV4 antibody and a plasma membrane marker (WGA; wheat germ agglutinin) (Ca). The TRPV4 signal within the plasmalemma is marked by arrowheads. Transfection with the Trpv4:eGfp construct (Cb). While TRPV4 expression is predominantly cytosolic, the TRPV4‐ir signal colocalizes with WGA at the plasma membrane (arrowheads). Scale bar = 50 μm. [Color figure can be viewed at wileyonlinelibrary.com]