A, TRPV4 colocalizes with blood vessel marker tomato lectin (TL). The lumen of the blood vessel is marked by the arrow. Putative RGCs are labelled by arrowheads. Scale bar = 20 μm. B, double labelling for TRPV4 and glutamine synthetase (GS); the two proteins colocalize in Müller end‐feet (arrowheads) and MVECs (arrow). Scale bar = 50 μm. C, TRPV4‐ir is reduced in retinas pre‐incubated with a blocking antibody. Scale bar = 20 μm. ILM, inner limiting membrane; RGCL, retinal ganglion cell layer; IPL, inner plexiform layer; INK, inner nuclear layer. [Color figure can be viewed at wileyonlinelibrary.com]