Table 2.
Host species, primary antibodt | Concentration | Company |
---|---|---|
Rabbit, TRPV4 | 1:2000 | LS‐A8583‐50; Lifespan Biosciences, Seattle, WA, USA |
Rabbit, VE‐cadherin | 1:500 | Sc‐28644; Santa Cruz Biotechnology; Santa Cruz, CA, USA; gift from M. E. Hartnett, University of Utah |
Rabbit, Occludin | 1:1000 | Sc‐5562; Santa Cruz Biotechnology; gift from M. E. Hartnett, University of Utah |
Mouse, β‐catenin | 1:1000 | Sc‐7199; Santa Cruz Biotechnology |
Rabbit, horseradish peroxidase | 1:2000 | Sc‐2004; Santa Cruz Biotechnology |
Mouse, GAPDH | 1:5000 | ab185059; Abcam, Cambridge, MA, USA |