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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Shock. 2017 Dec;48(6):666–673. doi: 10.1097/SHK.0000000000000907

Figure 1. Size-dependence of HS binding to CTH.

Figure 1

(a,b) Representative isotherms of calf thymus histone (CTH, 68.2 µM) binding to highly-sulfated HS oligosaccharides (400 µM). No binding was noted with HS tetrasaccharides (dp4, a); similarly negative isotherms were noted with dp6 and dp8 oligosaccharides. In contrast, fragments ≥ 10 saccharides (dp10, b) in size demonstrated both endothermic (downsloping) and exothermic (upsloping) binding reactions. (c) Isothermic calorimetry quantification of number of binding sites (N) and binding affinity of CTH-HS reactions.