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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Bone Miner Res. 2017 Aug 14;32(11):2182–2193. doi: 10.1002/jbmr.3224

Figure 1. Serum N-Formylkynurenine Levels Increase with Age.

Figure 1

Mice of the indicated ages were sacrificed and blood collected by cardiac puncture. Serum was prepared and flash frozen in liquid nitrogen. Frozen samples were thawed and analyzed by RP-HPLC for the levels of N-formylkynurenine (NFK) as described in Methods. The units for the MS measurements in the Y-axis are mAU (milli Absorbance Units). Relative concentrations were determined by injecting equal volumes of undiluted serum extractions for all mice of each age group. Values represent the mean ± SD of 10 animals of each age.