Effect of acetic acid on egg deposition over 24 h by
Drosophila. (A) Variation in oviposition with acetic acid
concentration in no-choice assay. ANOVA for acetic acid concentration:
F3,32 = 8.57, p<0.001). (B) Oviposition preference over
24 h between the control substrate (acetic acid-free food) and test substrate
containing acetic acid at three concentrations (the acetic acid-free substrate
treatment is the negative control, with mean value that does not differ
significantly from zero, p>0.05). ANOVA for oviposition index,
calculated as described in Methods section: F3,32 = 27.0,
p<0.001). Treatments with different letters are significantly different
by Tukey’s post hoc test.