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Polyacrylamide gel electrophoresis profile in 12% SDS-PAGE. a Non-reducing conditions. b Reducing conditions with β-mercaptoethanol. Lane MM) indicates the molecular weight marker. Lane 1: The protein isolates at pH 11, 4 treatment (40 μg/μl). Lane 2: Fractions > 10 kDa digested with trypsin for 2.5 h. Lane 3: Fractions > 10 kDa digested with chymotrypsin for 2.5 h; Lane 4: Fractions > 10 kDa digested with pepsin–trypsin for 2.5 h
