Fig. 4.

Effects of four fractions on the growth and survival of HeLa cells. The column graphs (a–e) showed the in vitro inhibition ratio of HeLa cells by four fractions at different concentrations for 24 h. The cell images (A–F) showed the cell growth morphology of A control group, B petroleum ether fraction (200 μg/mL), C ethyl acetate fraction (200 μg/mL), D n-butanol fraction (200 μg/ml), E water fraction (200 μg/mL) and F 5-fluorouracil (200 μg/mL), respectively, observed with invert microscope. Letters a–e refer to significant differences within a fraction/compound by one-way analysis of variance-Duncan’s multiple range test (p ≤ 0.05)