Fig. 5.

Effects of four purified compounds on the growth and survival of HeLa cells. The column graphs (a–e) showed the in vitro inhibition ratio of HeLa cells by four purified compounds at different concentrations for 24 h. The cell images (A–F) showed the cell growth morphology of A control group, B RHG (100 μg/mL), C calycosin (100 μg/mL), D formononetin (100 μg/mL), E rhamnocitrin (100 μg/mL) and F 5-fluorouracil (100 μg/mL), respectively, observed with invert microscope. Letters a-e refer to significant differences within a fraction/compound by one-way analysis of variance-Duncan’s multiple range test (p ≤ 0.05)