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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: J Immunol. 2017 Mar 3;198(7):2772–2784. doi: 10.4049/jimmunol.1600310

FIGURE 1.

FIGURE 1

StII does not show permeabilizing activity on liposomal vesicles composed of DPPC:Cho (1:1). LUVs comprising DPPC:Cho or PC:SM (1:1 molar ratio) encapsulating CF were exposed at different StII concentrations (2.5–1400 nM). LUVs were diluted in 10 mM Tris-HCl, 140 mM NaCl (pH 7.4) to reach a final lipid concentration of 0.2 µM. Vesicles of PC:SM were used as positive control. Excitation wavelength: 490 nm. Emission wavelength: 520 nm. The experiments were carried out at room temperature. The fraction of permeabilized vesicles (f) at a fixed time was estimated as: f = (Ff – F0)/(Fmax – F0), considering that release of fluorescent probe is simultaneous to pore formation, where F0 and Ff are fluorescence values before and after adding StII, respectively, and Fmax is the fluorescence measured in the presence of 0.1% Triton X-100. Data are from a single experiment representative of two independent experiments.