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. 2017 Nov 14;7:15573. doi: 10.1038/s41598-017-15828-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prep1 down-regulation increases C/EBPβ binding to chromatin in 3T3-L1 cells without affecting C/EBPβ level and phosphorylation. (A) Immunoblotting analysis of cell extracts from P and C cells at various time points after induction using anti-phospho-C/EBPβ (Thr235), and total C/EBPβ Abs, recognizing three isoforms of C/EBPβ - LAP*, LAP, LIP. Vinculin Ab was used as loading control. (B) Venn diagrams of C/EBPβ binding peaks in Prep1 down-regulated (P) and control (C) cells before (−2 d) and 4 hours (4 h) after the induction of differentiation. (C) Venn diagrams and Gene Ontology analysis of genes bound by C/EBPβ in P and C cells before (−2 d) and 4 hours (4 h) after induction. (D) DNA-binding motifs (Cebpβ and AP1) of C/EBPβ binding sites in P and C cells before (−2 d) the induction of differentiation, depicting common and exclusive peaks.